This study demonstrates that exposure of cells to extremely low-frequency electromagnetic fields can cause measurable changes in protein synthesis. Sciara coprophila salivary gland cells were exposed to five low-frequency (1.5-72 Hz) electromagnetic signals: three signals (1.5, 15, and 72 Hz) produced pulsed asymmetric electromagnetic felds and two signals (60 and 72 Hz) were sinusoidal. Subsequent analyses of two-dimensional gels showed that cell exposure to either type of low-frequency electromagnetic field resulted in both qualitative and quantitative changes in patterns of protein synthesis. Thus, signals producing diverse waveform characteristics induced previously undetectable polypeptides, some of which were signal specific and augmented or suppressed other polypeptides as compared with nonexposed cells. The pattern of polypeptide synthesis differed from that seen with heat shock: only five polypeptides in cells exposed to electromagnetic signals overlap those polypeptides exposed to heat shock, and the suppression of protein synthesis characteristic of heat shock does not occur.analysis of radioactive uridine uptake into molecular size classes expected of mRNA species (6, 7); mRNA with sedimentation coefficients of 6-10S and 20-25S was augmented or induced relative to mRNA from control cells. Most new or augmented mRNA was polyadenylylated, based on binding to oligo(dT)-cellulose columns under hybridization conditions. In this study, a more sensitive assay of determining alterations in the pattern of polypeptide synthesis in exposed cells was used. The results showed that exposure of salivary gland cells to elf electromagnetic signals with diverse waveform characteristics and shapes caused the appearance of previously undetected polypeptides, some of which were signal specific and altered synthesis in other polypeptides from that seen in control cells. Five polypeptides associated with exposure to elf electromagnetic fields were those seen under heat-shock conditions.
Xanya Sofra Weiss
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