K. Macfelda, B. Kapeller, U. M. Losert; 2005Background: Myocardial cell transplantation in patients with heart failure is emerging as a potential therapeutic option to augment the function of remaining myocytes. Nevertheless further investigations on the basic issues such as ideal cell type continues to be evaluated. Therefore, the aim of our study was to compare the performance of skeletal muscle cells and cardiomyocytes in respect of their proliferation rate and viability on different extracellular matrix components (EMC).
Methods: Rat cardiomyocytes (RCM) and rat skeletal muscle cells (RSMC) were cultured on EMC such as collagen type I, IV, laminin, fibronectin, and gelatine. The components were used as “double coating”. Proliferation rates were determined by proliferation assays on day 1, 2, 4, and 8 after inoculation of the cells.
Results: The most essential result is that collagen type I enhances the proliferation rate of RSMC but decreases the proliferation of RCM significantly. This effect is independent of the second EMC used for the double-coating studies. Other EMCs also influence the cellular behaviour, whereas the sequence of the EMCs is essential.
Conclusions: Results obtained with our studies reveal the significantly different proliferation behaviour of RCM and RSMC under identical conditions. As skeletal muscle cells are also used in heart tissue engineering models, these results are essential and should be investigated in further studies to prove the applicability of skeletal muscle cells for heart tissue engineering purposes.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment